MALDI - TOF VITEK MS for the rapid and accurate
نویسندگان
چکیده
Laboratory of Applied Microbiology, University of Applied Sciences and Arts of Southern 12 Switzerland, Bellinzona, Switzerland; bioMérieux SA, Unit Microbiology, R&D 13 Microbiology, La Balme Les Grottes, France; Department of Dermatology, University 14 Hospital of Zurich, Zurich, Switzerland; Microbiology and Virology Unit, Papa Giovanni 15 XXIII Hospital, Bergamo, Italy; POLE Pharma Consulting , Breganzona, Switzerland 16 17 18 Address correspondence to [email protected] 19
منابع مشابه
Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry for identification of Clostridium species isolated from Saudi Arabia.
The aim of this study was to identify different Clostridium spp. isolated from currency notes from the Ha'il region of Saudi Arabia in September 2014 using MALDI-TOF-MS. Clostridium spp. were identified by Bruker MALDI-TOF-MS and compared with VITEK 2. The confirmation of the presence of different Clostridium spp. was performed by determining the sequence of the 16S ribosomal RNA gene. In this ...
متن کاملCharacterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES).
BACKGROUND/AIM The purpose of the study was to evaluate the performance of the VITEK mass spectrometry (MS) (bioMérieux, France) system for the identification of Klebsiella spp. isolated from different sources. Moreover, while assessing the ability of the VITEK 2 automated expert system (AES) to recognize antimicrobial resistance patterns, the researchers have extended the study to compare VITE...
متن کاملPerformances of the MALDI-TOF Mass Spectrometry system
22 23 Rapid and cost-effective MALDI-TOF MS-based systems will replace conventional 24 phenotypic methods for routine identification of bacteria. We report here the first evaluation 25 of the new MALDI-TOF MS-based VITEK MS system in a large clinical microbiology 26 laboratory. This system used an original spectra classifier algorithm and a specific database 27 designed for the identification o...
متن کاملRoutine identification of Nocardia species by MALDI-TOF mass spectrometry.
We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The...
متن کاملRapid identification of mycobacteria from positive MGIT broths of primary cultures by MALDI-TOF mass spectrometry
BACKGROUND Rapid identification of mycobacteria is important for timely treatment and the implementation of public health measures. The MGIT system ensures rapid detection of mycobacteria, but identification is usually delayed by days to weeks due to further subculture on solid medium. Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to effective...
متن کاملEvaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi.
Invasive fungal infections have a high rate of morbidity and mortality, and accurate identification is necessary to guide appropriate antifungal therapy. With the increasing incidence of invasive disease attributed to filamentous fungi, rapid and accurate species-level identification of these pathogens is necessary. Traditional methods for identification of filamentous fungi can be slow and may...
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